Description

Overview:

Lysozyme is a glycoside hydrolase, consisting of 18 kinds of 129 amino acid residues, with 4 S-S bonds and a molecular weight of 14307. It is a hydrolase that acts exclusively on the cell wall of microorganisms. In the secretion fluid and some plants and microorganisms, it can dissolve the cell wall of Gram-positive bacteria and has a strong killing effect on it. , sake production), medicine (tablets, capsules, eye drops, throat lotion), daily chemicals (toothpaste, cosmetics), baby food (breast milk powder) and scientific research has a wide range of applications.

Features:

1. As a natural preservative, lysozyme has unique advantages such as strong antibacterial properties, safety and non-toxicity, good thermal stability and wide range of action.

2. Lysozyme is a kind of protein, which is relatively stable to pH changes and thermally stable under acidic conditions. By adopting bioengineering technology, adjusting protein PH value and using ion exchange resin to adsorb and separate spermase, the enzyme activity is greater than 18,000 u/g, reaching the standard of pharmaceutical grade.

3. Lysozyme can dissolve bacterial cells, especially gram-positive bacteria, and has various properties of antibiotics. Decomposition can decompose chitin and ethylene glycol chitin to obtain carbonate, hydrochloride and nitrate crystals.

Product properties: light yellow or white solid powder

Product specification: food grade or pharmaceutical grade 20,000 u/mg

Other customized specifications: 1000U/mg, 5000U/mg, etc.

Application areas:

1. Lysozyme is a non-toxic, no side effect protein, and has a certain bacteriolytic effect, so it can be used as a natural food preservative. It has been widely used in the preservation of aquatic products, meat products, cakes, sake, cooking wine and beverages; it can also be added to milk powder to emulsify milk to inhibit the survival of spoilage microorganisms in the intestinal tract, and at the same time directly or indirectly promote the Proliferation of bifidobacteria in the gut.

2. As a non-specific immune factor existing in normal body fluids and tissues of the human body, lysozyme has a variety of pharmacological effects. It has antibacterial, antiviral and antitumor effects. At present, the indications of medical lysozyme are bleeding and hematuria. , bloody phlegm and rhinitis.

3. Lysozyme has the function of destroying the structure of bacterial cell wall. This enzyme treats G+ bacteria to obtain protoplasts. Therefore, lysozyme is an indispensable tool enzyme for cell fusion operations in genetic engineering and cell engineering.

Mechanism of action:

Lysozyme can effectively hydrolyze peptidoglycan of bacterial cell wall, and its hydrolysis site is the β-carbon atom between the 1st carbon atom of N-acetylmuramic acid (NAM) and the 4th carbon atom of N-acetylglucosamine (NAG). 1.4 Glycosidic bond. Peptidoglycan is the main component of bacterial cell wall, it is composed of NAM, NAG and peptide "tail" (usually 4 amino acids), NAM and NAG are connected by β-1.4 glycosidic bond, and the peptide "tail" is connected by D- The lactyl carboxyl group is connected to the 3rd carbon atom of NAM, and the peptide tails are connected by a peptide "bridge" (peptide bond or a few amino acids), and NAM, NAG, peptide "tail" and peptide "bridge" together form a The multi-layered network structure of peptidoglycan acts as the skeleton of the cell wall, and any chemical bond breakage in the above structure can lead to damage to the bacterial cell wall. For gram-positive bacteria (G+), such as Micrococcus luteus, Bacillus subtilis or Micrococcus lysolyticus, etc., and Gram-negative bacteria (G-), such as Escherichia coli, Proteus, Shigella, Pneumococcus, etc., The content of peptidoglycan in the cell wall is different. The cell wall of G+ bacteria is almost entirely composed of peptidoglycan, while the inner wall layer of G- bacteria is only peptidoglycan. Therefore, lysozyme is stronger than G- bacteria for destroying the cell wall of G+ bacteria.

Conditions of Use:

As a typical representative of lysozyme, egg white lysozyme is a stable protein with an optimum pH of 4-6.5 and an optimum temperature of 35°C. The optimum amount of enzyme added must be determined according to the specific production situation. Details Please write or call for consultation.

store:

It is recommended to store in a cool and dry environment away from light. Storage temperature: below zero.

Long-term storage or unfavorable storage conditions will reduce the enzyme activity to varying degrees; if the temperature and humidity are too high, it is necessary to appropriately increase the usage amount during use.

Safety:

Lysozyme is a pure natural enzyme preparation, which is a protein. The food that eats the enzyme preparation is like eating the food containing protein, which is beneficial and harmless to the human body.

For some sensitive people, such as direct ingestion of highly concentrated enzyme powder or droplets, it may cause allergies, and prolonged contact may irritate the skin, eyes and mucous membranes. It is recommended to wear protective equipment such as masks and goggles during the operation. The remaining or spilled enzyme powder should be disposed of in time. For a large amount of enzyme powder spilled, it should be gently swept back to the container, and a small amount should be vacuumed or soaked with water for cleaning. 

Lysozyme is a biologically active substance that is easily inhibited and destroyed by heavy metal ions (Fe3+, Cu2+, Hg+, Pb+, etc.) and oxidants, and should be avoided during storage or use.

Package:

Packed in aluminum-plastic bag, 0.5kg×10 bags/carton; 0.5kg×20 bags/carton